Hyaluronic Acid is used as As an endogenous skin protection factor, it can promote the nutrition metabolism of the skin, make the skin moist, fresh and elastic, and play the role of anti-wrinkle, anti-wrinkle, delaying the aging of the whole skin, and beautifying and beautifying the skin.Therefore, hyaluronic acid is the ideal product for improving dry and aging skin.
|Product Name||Hyaluronic Acid|
|Other Name||Hyaluronic Acid Sodium Salt;|
|Appearance and properties||White powder|
|Storage conditions||Stored in normal temperature|
Hyaluronic Acid is used for intermediates of making young skin.
Hyaluronic Acid was produced by the followings:
1. Add the crested pieces to acetone and soak overnight until the crested pieces become hard due to dehydration.Then placed in the porcelain plate for drying, after drying by a pulverizer into powder.Pour the comb powder into the enamel pot, add 6 ~ 7 times distilled water, stir evenly, soak for more than 24h, make the comb powder fully swell, then filter, collect filtrate, filtrate residue soakfor 3 times in the same way, combine filtrate for 3 times.Then pour the filtrate into the ceramic cylinder, add 10% solid sodium chloride after stirring, and add equal volume of chloroform. After stirring for 3h, separate the aqueous phase.Add 95% ethanol of 2 times volume into the separated aqueous phase, stir evenly, let stand overnight, precipitate out hyaluronic acid, then drain the sediment, and get the coarse product after drying.The coarse product dissolves again in 4 times volume
0 1mol/L sodium chloride solution, dilute with hydrochloric acid to adjust pH=
4, 5-5, then add the same volume of chloroform, after standing stratification, the upper water phase is sucked out.The aqueousphase uses 8% sodium hydroxide solution to adjust pH=
7 5, add the chain enzyme protease, heat preservation at 37℃ for 24h.The same volume of chloroform extraction was used for two times to combine the upper aqueous phase and the lower liquid to recover chloroform.Add 1% cetylpyridine chloride solution of the same volume into the aqueous phase, stir evenly, settle, filter and collect the precipitation.To precipitate by two or three times the volume
4mol/L sodium chloride was dissolved, filtered, and a volume of 95% ethanol (3 times) was added into the filtrate. After standing precipitation, the upper layer of ethanol was absorbed, and the lower layer was repeatedly precipitated for 2 times with 95% ethanol. The lower layer was suspended for filtration, dried, and then washed with acetone for 2 times, and then dried in vacuum.
2. Microorganisms (Streptococcus epizoides HA produces mutant Y9
21) Fermentation method.